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S1 (2020): S54S57

In homeostatic conditions, the association of pericytes and ECs decreases paracellular migration of peripheral immune cells and other inflammatory factors and supports the structure and maintenance of the BBB (Daneman and Prat, 2015

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To prepare 10 mM HCl: Take 1 mL of standard concentrated HCl (12 M) Add to 1,199 mL of sterile water yields 10 mM HCl Alternatively, purchase pre-prepared sterile 10 mM HCl or use a validated dilution from a laboratory stock solution Alternative Solvent: 1% Acetic Acid in Sterile Water Some protocols use 1% acetic acid (glacial acetic acid diluted in sterile water) as the initial reconstitution vehicle