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Since the waveguide is designed to perform effectively under varying light conditions, from low light with small aperture sizes (e.g., 0.2 mm) to larger apertures, we opted to proceed with experiments using three epoxy-filled cones

Luciferase assay PGL6-VGF-Luc or PGL6-gcg-Luc plasmids and Renilla luciferase expression plasmids were co-transfected into TC1-6 cells using Lipofectamine 2000 (Thermo Fisher, 11668), according to the manufacturers instructions

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