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Blood samples of children with Down syndrome and their siblings were collected by venipuncture and were analyzed blind by the Genetic Laboratory of the Faculty of Medicine of Lisbon, between the 1st of April of 1995 and the 1st of April of 1996

In more intensive therapy scenarios (such as for chronic fatigue or recovery support), a practitioner might recommend a series of daily injections over 5-10 days at 100-250 mg per day, then taper to a maintenance schedule

Fast for at least 2 hours before injection

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