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We have applied IMPACT in several different ways, including exploiting the fluorescent lipid products from IMPACT as tracers for imaging interorganelle phospholipid transport, 468,469 as reporters of the spatiotemporal dynamics of Gq-coupled GPCR signaling, 470 and as the readout in genome-wide CRISPR interference screens to elucidate regulators of protein kinase CPLD signaling

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