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preparation of compound 4: after being activated, the compound 3 and the amino at the end position of Arg34GLP-1 (9-37) in the compound 2 are subjected to acylation coupling reaction under the action of a condensation reagent and alkali to obtain a compound 4

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, CEPS, a promising green alternative, employs water-based conditions for fragment coupling instead of organic solvents such as those used in LPPS

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