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Tissue fixation and preparation of paraffin-embedded, frozen, and vibratome-cut sections Brain hemispheres were fixed by immersion in 4% paraformaldehyde in phosphate buffer solution (Nacalai tesque, Kyoto, Japan)

Participants included women at least 21 years old who were nonpregnant, had persistent sexual desire or arousal issues for at least six months, and demonstrated a prior history of sexual functionality spanning at least two years

The mobility of the population as well as the inability to precisely estimate exposure to ozone and other potential confounders over a period of many years degrades the power of, and leads to bias in, both longitudinal and cross-sectional studies

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