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ROS can directly interact with the subunit of PI3K, enhancing its activity and promoting the conversion of PIP2 to PIP3 (92)

This fact suggests that during this period, greater cellular activity is needed, the mitochondria are more active, and more ROS are generated

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The quantification methods include antioxidant assays (e.g., Glutathione assay, Ascorbic acid assay, Antioxidant enzyme activity assay), DNA damage/repair (e.g., 8-OH Guanine, double-strand break assay), peroxidation of lipids (e.g., 4-hydroxynonenal assay, malondialdehyde assay), ROS assay (e.g., H 2 O 2 assay, NO assay), protein modification (e.g., Advanced glycation end products, protein carbonyl assay), and Oxidase/Peroxidase activity (e.g., Monoamine oxidase assay, peroxidase/H 2 O 2 assay)