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To contextualise the weight reduction outcomes, NNTs were produced and are presented in Supplementary Material Table S10

We selected pAcF as the handles for constructing conjugates instead of other chemistry such as azide mediated click chemistry as the cyclopropane-tetrazine ligation employed in the pAcF system demonstrates superior bioorthogonality compared to azide-alkyne cycloadditions, effectively minimizing off-target reactions in complex cellular environments

Tirzepatide is approved for managing type 2 diabetes and is also approved for chronic weight management in adults with obesity or overweight (similar to Semaglutide)

Symptoms of too little thyroid hormones (hypothyroidism) include: Weight gain Trouble tolerating cold Constipation Fatigue or excessive tiredness Joint pain Muscle aches or pain Dry skin Thinning hair Slowed heart rate Heavy menstrual cycles Irregular menstrual cycles Feeling depressed more often than usual Symptoms of too much thyroid hormone (hyperthyroidism) are: Weight loss combined with eating more than usual Irregular, fast heartbeat Shaky hands Muscle weakness Trouble sleeping Fatigue Irritability Feeling anxious more often than usual Trouble adjusting to warm weather Too many bowel movements A goiter, which is an enlargement of the neck Weight loss is a main impact of GLP-1 medications, which may make it difficult to tell when a decrease on the scale stems from the weight loss medication or disruptions to the thyroid hormones in the body