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Most protocols require reorders every 46 weeks depending on dose frequency

To block nonspecific binding, after PBS washing, normal animal non-immune serum was applied, and the sections were incubated at room temperature for 1 h, followed by gentle tapping to remove excess serum

Importantly, MK2 inhibition effectively suppressed HSP27 phosphorylation, including in MAPKAPK2-overexpressing cells, confirming successful blockade of MK2 kinase activity (Figure 4B)

10.1016/j.neurobiolaging.2016.08.019 69 CekanaviciuteE.YooB