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Peptides were precipitated with cold (4 C) diethyl ether and purified on an Agilent 1200 series High-Performance Liquid Chromatography (HPLC) instrument (1075% HPLC-grade acetonitrile for 20 min at a 2 mL/min flow rate over an Agilent Zorbax C18 column (5 m, 9.4 250 mm) tracked at 220, 254, and 280 nm

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Of them, sodium ferulate (SF) features low toxicity, stability, ease of synthesis, and water solubility and has been authorized by the State Drug Administration of China as an adjuvant drug for treating ischemic cardiovascular diseases [166, 167]