GSH is involved in the detoxification of free radicals, peroxides and xenobiotics (drugs, pollutants, carcinogens, etc.), protects biological membranes from lipid peroxidation, and is an important regulator of cell homeostasis, since it participates in signaling redox, regulation of the synthesis and degradation of proteins (S-glutathionylation), signal transduction, various apoptotic processes, gene expression, cell proliferation, DNA and RNA synthesis, etc
Validation The current study used the same enzymatic samples to assess the accuracy of a modified GPx-DTNB method compared to the original GPx Assay (Rotruck et al
Early response predicts long-term success
The components that were retained were 10 mM TrisCl, pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, and 1 mM PMSF