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Primary human hepatocytes (PHH) were obtained from BioIVT (donors IAN, IPH, GID) and from Lonza (donors HUM4108, HUM4055B, HUM4229, HUM181501B)

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Use this method only occasionally and always consult a healthcare provider before trying it

The planned outcome came out to be a pH of 11, a temperature for extraction of 47 C, a solution in which the mass of the solid is one-third the volume the volume of the solvent, and a surfactant concentration of 0.1 mol/L sodium chloride