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Interestingly, notable differences were observed in these sites between the two receptor states: for the active state, site I (purple) was located in the upper TM1 and TM7 region, while in the inactive state, site I (cyan) was found in the lower TM5 and TM6 region, an area which undergoes key structural changes upon G protein engagement and GLP-1R activation (30) and might therefore represent a state-specific cholesterol binding site