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Sci Rep (2016) 6

Each time a cell divides, telomeres shorten, eventually triggering cellular senescence or apoptosis - effectively limiting the replicative lifespan of normal somatic cells to approximately 50-70 divisions (the Hayflick limit)

By comparing the metabolic pathway activities for the gdh3 / gdh3 mutant grown on arginine medium as opposed to on proline medium with the wild-type strain under those growth conditions, there was a greater global metabolic reprogramming for the wild-type cells when grown on arginine compared to growth on proline (Fig

Cells were washed with PBS and blocked with 5% BSA for 1 h at RT