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But theres a significant gap in human clinical evidence

20 ng of cDNA was used per qPCR reaction using Powerup SYBR green master mix (Thermo Fisher Scientific) and gene specific primers according to manufacturers instructions on a Quantstudio 3 Real-Time PCR system (Thermo Fisher Scientific)

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3 Clinical implications and treatment perspectives Recent data support integrating routine metabolic screening, such as fasting blood glucose, fasting insulin, and homeostatic model assessment of IR (HOMA-IR), into standard BD care to identify high-risk individuals