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Samples were reduced with DTT and alkylated with iodoacetamide as described above, then diluted with DPBS (300 L), and taken forward to LysC and trypsin digestion without precipitation

Both peptides must be reconstituted with bacteriostatic water (not sterile water) and stored at 28C in a refrigerator immediately after mixing

Pinch a fold of skin and fatty tissue, insert the needle at a 45-degree angle (rather than straight down), inject slowly over 35 seconds to allow tissue to accommodate the fluid gradually, and withdraw the needle slowly

Patients turn the dose selector until the desired dose (0.25 mg, 0.5 mg, 1 mg, or 2 mg) aligns with the dose pointer in the window