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The enzyme is complex, with four copper atoms, two or more disulfides, glycosylation, optimal activity at acidic pH (pH 45) and an electron transfer pathway that is strongly interrupted by modest concentrations of hydroxyl anions or halides (for example, NaCl and KCl) in cell culture media 21

This effect, mediated by inactivation of the phosphoinositide 3-kinase activation (PI3K/AKT) and extracellular-signal-regulated kinase (ERK) signaling pathways, offers an insight into the complex mechanisms of miscarriage and preeclampsia during Se deficiency

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