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Although NRPS product assembly frequently seems to display a strict correlation between the enzymatic domain sequence of the megasynthase and the position of the amino acid building blocks in the peptide product, this colinearity rule is not strictly reinforced in nature 36,37
Manipulation and mobilization for treating chronic low back pain: A systematic review and meta-analysis
Therefore, HPLC gel filtration is recommended as a final polishing step that will isolate properly folded protein from aggregates or other contaminating species
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