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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)

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This therapeutic effect is associated with enhanced intestinal barrier function, achieved through modulation of the intestinal NLRP3 inflammasome, highlighting a novel pathway through which acupuncture may confer systemic neuroprotection