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These studies may be complemented by temperature control technologies (e.g., localized heating or the use of temperature-tolerant strains) to better approximate human physiological conditions (Renshaw and Trede, 2012

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Cells were added to the apical chamber of 1% Matrigel-coated transwells in 100 L of proliferation media for the 24-well transwells or to the 5% Matrigel-coated well in 250 L proliferation media for 48-well plates