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If you miss a dose, take it as soon as you remember

doi: 10.1146/annurev-animal-020518-115206 15 Snchez-CastilloAHeylenEHounjetJSavelkoulsKGLieuwesNGet al

Cells were permeabilized with 0.2% Triton X-100 and 1 mg/ml lysozyme, and probed with affinity-purified anti-GspD antibody at a 1:10 dilution in PBS buffer with 2% BSA

Non-specific binding was blocked using serum from the species in which the secondary antibody was raised