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Mistake 10: reconstituting more than you can use If your protocol calls for a 28-day supply and your vial contains enough peptide for 60 days of dosing at your chosen concentration, do not reconstitute the entire vial at once
Ultrastructural analysis revealed that the mitochondrial ridge was reduced or absent and that rupture of the outer mitochondrial membrane (OMM) occurred in the Erastin-treated group and sh-GPX4 group(Fig
Always label vials with the date of first puncture
The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software
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