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In many experimental animal models, MTX has been found to increase serum urea and creatinine levels (Reference Moodi, Hosseini and Abedini16,Reference Tousson, Zaki and Abu-Shaeir50,Reference Jafaripour, Naserzadeh and Alizamani51) , but there are also studies showing that MTX reduces serum urea concentration (Reference Budancamanak, Kanter and Demirel52Reference Severin, Campagno and Brandoni54)

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Muoz-Caro T, Gibson AJ, Conejeros I, Werling D, Taubert A, Hermosilla C (2021) The role of TLR2 and TLR4 in recognition and uptake of the apicomplexan parasite eimeria bovis and their effects on NET formation

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