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In vitro reactive oxygen species (ROS) assay ARPE-19 cells were cultured as above then seeded at a concentration of 2.5 10 4 cells/well (200 L) in fresh culture media in 96-well black walled, clear bottom tissue culture treated plates for fluorescence detection

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We found that component anwuligan in forsythia may activate the Wnt/-catenin signaling pathway by binding to CTNNB1