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Based on this, n = 34 mice per group was considered appropriate to detect treatment-associated trends while complying with ethical principles for animal research

However, long-acting GCG receptor (GCGR) agonists have also been shown to dose-dependently stimulate insulin secretion and enhance energy expenditure and fatty acid oxidation, leading to substantial weight loss in rodents [4, 5]

"Aggregation Behavior of Structurally Similar Therapeutic Peptides Investigated by 1H NMR and All-Atom Molecular Dynamics Simulations." Molecular Pharmaceutics, 2022

For cells, they were first washed with PBS and fixed at room temperature for 15 min, followed by incubation with the staining solution at pH 6.0 overnight at 37 C