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DPP-4 enzyme assays The enzyme assay was performed in flat-bottom 96- well microplates, where each reaction consisted of 100 L of either 12.5% human, platypus or echidna serum, 0.5 mM of H-Ala-Pro-p-nitroanilide (Bachem) as a substrate in the presence/absence of 100 M of DPP-4 inhibitor P32/98 (Santa Cruz Biotech) in 100 mM sodium phosphate buffer (PH 7.4) supplemented with 150 mM NaCl and 0.2% Tween-20 with a total volume of 200 M

GLP-1/GIP, GLP-1/glucagon, and even triple agonists are the dominant focus, though the spectrum extends to other mechanisms as wellamylin, GIP antagonism, NK2 receptor agonism, and more, said DiMarchi Looking further ahead, DiMarchi sees potential for oral therapies, including oral peptides

TAKEAWAYS Weve reviewed many situations where insulin still plays a significant role in diabetes care