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MINFLUX microscopy INS-1 832/3 SNAP/FLAG-hGLP-1R cells were seeded onto 18-mm coverslips, labelled with SNAP-Surface Alexa Fluor 647 and stimulated with 100 nM exendin-4 as above

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The first sub-analysis involved stratifying patients based on their AHM initiation status: newly-initiated users (patients who started AHMs on or after January 1, 2011, with no prior recorded use) and prevalent users (patients with a history of AHM use before January 1, 2011)