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Using immobilon chemiluminecent HRP substrate (Millipore, Massachusetts, USA), the protein bands were visualized with the Tanon-5200 Chemiluminescent Imaging System (Tanon, Shanghai, China)

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By inhibiting this enzyme, NAD+ levels rise within cells, leading to enhanced energy metabolism , lipid breakdown , and mitochondrial activation

Nonexpression of GLP-1R is common in advanced pancreatic tumors with lymphatic metastasis and is associated with poor prognosis, suggesting that GLP-1R may inhibit tumorigenesis and metastasis of human pancreatic cancer cells both in vitro and in vivo