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Instead, choose a spot at least two inches away to prevent irritation and ensure proper absorption

Samples were separated using a Raptor HILIC-Si 2.1 100 mm, 2.7 m LC column (Restek) with a constant flow rate of 400 l/min following a flow gradient starting at 5% Mobile Phase B (H 2 O + 5 mM AmFm +0.5% formic acid) then going up to 40% in 5 min, which was followed by re-equilibration at 5% B for 4 min and 30s