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The explanation for this discrepancy can be found in recent papers showing that some cancer cell linesincluding HCT116are not affected by L-BSO or GSH synthesis inactivation because they can compensate GSH depletion by the thioredoxin (TXN) pathway 25 , and by maintaining protein homeostasis through deubiquitinating enzymes (DUB) 32

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Following each experiment, the cells were washed once with PBS (LPS solution CBP007B) and fixed with 10% formalin solution (Sigma HT501128-4L) for 10 min at room temperature

Canonical Wnt/-catenin signaling pathway is activated via increased production of Wnt ligands by NTAPP To investigate whether NTAPP exposure affect a biological response via the canonical Wnt/-catenin signaling pathway, we treated hDP cells with two types of pharmacological inhibitor of Wnt/-catenin signaling, endo-IWR1 25 and IWP2 26 before NTAPP treatment