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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

To assess the metabolic stability of the synthesized peptides, serum metabolism studies were conducted using blood obtained from 4-month-old male Sprague-Dawley rats

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Further diagnostic investigation for thrombocytopenia relies on a detailed review of the other values on the CBC (red cell count, hemoglobin, white blood cell count, mean platelet volume or MPV), the comprehensive blood chemistry panel (kidney function, liver function, electrolytes), blood coagulation panel (other components of the clotting system), and review of the blood smear under the microscope (looking for fragmented red cells, shape and size of white cells, red cells, platelets)