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Expression of Adcy 1 (NM_009622.1), Adcy 2 (NM_153534.2), Adcy 3 (NM_138305.3), Adcy 4 (NM_080435.1), Adcy 5 (NM_ 001012765.4), Adcy 6 (NM_007405.2), Adcy 7 (NM_ 001037723.3), Adcy 8 (NM_009623.2), Adcy 9 (NM_ 009624.2), and -actin (NM_007393.3) mRNAs were determined by real-time PCR using an Applied Biosystems TaqMan Fast Universal Master Mix and gene specific TaqMan primer probe sets (Adcy 1, Mm01187829_ m1

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If a day is missed, the research-sensible response is simply to resume the next scheduled dose, not to double up, because there is no acute deficit to make good and doubling only departs from the documented figure

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