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Supplemental 25-Hydroxycholecalciferol Is more effective than cholecalciferol in raising serum 25-Hydroxyvitamin D concentrations in older adults

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Recombinant thioredoxin was reduced by 5 mM DTT for 30 min, desalinated via a micro bio-spin column with Bio-Spin p-6 desalinating columns (Biorad, Munich, Germany), and re-formation of disulfide bonds was prevented by treating it with 10 mM iodoacetic acid