Briefly, GSH assay buffer, GSH reductase, 5,5-dithio-bis 2-nitrobenzoic acid solution and supernatant sample were mixed together and incubated at 25C for 5 minutes, then NADPH was added into this system to trigger the reaction
Witte AB, Anestl K, Jerremalm E, Ehrsson H, Arnr ES (2005) Inhibition of thioredoxin reductase but not of glutathione reductase by the major classes of alkylating and platinum-containing anticancer compounds
Treatment with FOXO4-DRI following chemotherapy exposure potently counteracts these effects, normalizing body weight and reducing plasma AST to near-baseline levels
Research Considerations Across the preclinical literature, BPC-157 is consistently described as well tolerated with no established lethal dose in animal toxicology, and injection-site reactions among the few commonly noted effects