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Hyrich, K

Structure of the micro-opioid receptor-Gi protein complex

After three washes with PBS, the cells were treated with 0.1% NaBH 4 in PBS for 7 min at room temperature

The results of immunostaining and western blot showed that an obvious increased protein expression of 4-HNE was found in peroxynitrite treated SGNs, while it was significantly decreased in peroxynitrite + ebselen group but further increased in peroxynitrite + gpx1 / mice compared with the peroxynitrite-only group (Figures 5B,C)